GSTrap Fast Flow, Cytiva, 17-5130-02, pack of 2 x 1 mL

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

GST*-tagged Proteins can be purified directly from pretreated bacterial lysates using GSTrapTM FF.GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods. The medium, Glutathione SepharoseTM 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTATM design or FPLCTM System.

Features and Benefits

Fast and simple one-step purification of GST-tagged Proteins. Mild elution conditions preserving Protein antigenicity and function. Easy scale-up by connecting the columns in series. Sepharose^® 4 Fast FLow provides good fLow properties.

General description

GSTrap^™ FF columns are prepacked Glutathione Sepharose^® Fast FLow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

Legal Information

Sepharose is a registered trademark of Cytiva

GSTrap is a trademark of Cytiva

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)